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Image Search Results
Journal: Cells
Article Title: The Impact of ALS-Associated Genes hnRNPA1 , MATR3 , VCP and UBQLN2 on the Severity of TDP-43 Aggregation
doi: 10.3390/cells9081791
Figure Lengend Snippet: SH-SY5Y cells co-transfected with mKO2 constructs and wt or mut UBQLN2. ( A ) Quantification of co-transfected cells harboring aggregates. ( B ) Average number of aggregates in an individual cell. ( C ) Average aggregate size. ( D ) SH-SY5Y cells co-transfected with mKO2 constructs and wt or mut UBQLN2. Probed for HA-tag and counterstained with DAPI. Scale bars: 10 µm.
Article Snippet: Then, four ALS-linked genes were amplified by PhusionTM High-Fidelity DNA Polymerase and inserted into linearized pcDNA5/FRT/TO-3xHA (XhoI (Thermo Fisher Scientific) and EcoRV (Thermo Fisher Scientific)) by CloneEZ PCR Cloning Kit: VCP (Addgene #23971, deposited by Nico Dantuma [ ]),
Techniques: Transfection, Construct
Journal: Cells
Article Title: The Impact of ALS-Associated Genes hnRNPA1 , MATR3 , VCP and UBQLN2 on the Severity of TDP-43 Aggregation
doi: 10.3390/cells9081791
Figure Lengend Snippet: Summary of TDP-43 aggregation strains in co-transfected SH-SY5Y cells. Full red shapes represent TDP-43 aggregates, twisted lines represent soluble TDP-43, and orange and yellow shapes represent TDP-43 aggregates co-localized with UBQLN2.
Article Snippet: Then, four ALS-linked genes were amplified by PhusionTM High-Fidelity DNA Polymerase and inserted into linearized pcDNA5/FRT/TO-3xHA (XhoI (Thermo Fisher Scientific) and EcoRV (Thermo Fisher Scientific)) by CloneEZ PCR Cloning Kit: VCP (Addgene #23971, deposited by Nico Dantuma [ ]),
Techniques: Transfection